Angelini Lab | Biomolecular Engineering

Publications

Mazzocato Y., Frasson N., Sample M., Fregonese C., Pavan A., Caregnato A., Simeoni M., Scarso A., Cendron L., Šulc P. and A. Angelini. “Combination of co-evolutionary information and supervised learning enables generation of cyclic peptide inhibitors with enhanced potency from a small dataset”. ACS Central Science. 10, 2242-52

Yamamoto Y., Taniguchi H., Nguyen N.M., Yokoyama F., Choowongkomon K., Angelini A., Horiuchi J. and Y. Kumada. “Development of a novel and broadly applicable sandwich ELISA assay based on rabbit single-chain variable fragments and a modified Ig-binding domain of protein L fused to a polystyrene-binding peptide”. Journal of Immunological Methods. 534, 113771

Linciano S., Vigolo E., Rosato A., Kumada Y. and A. Angelini (2024). “Albumin-based strategies to effectively prolong the circulation half-life of small immunomodulatory payloads in cancer therapy”. Current Opinion in Biotechnology. 90, 103218

Nielsen A.L., Bognar Z., Mothukuri, G.K., Zarda A., Schüttel M., Merz M.L., Ji X., Will E.J., Chinellato M., Bartling C.R.O., Strømgaard K., Cendron L., Angelini A. and C. Heinis (2024). “Large Libraries of Structurally Diverse Macrocycles Suitable for Membrane Permeation”. Angewandte Chemie International Edition. e202400350

Chinellato M., Perin S., Carli A., Lastella L., Biondi B., Borsato G., Di Giorgio E., Brancolini C., Cendron L. and A. Angelini(2024). “Folding of class IIa HDAC derived peptides into a-helices upon binding to myocyte enhancer factor-2 in complex with DNA”. Journal of Molecular Biology. 436, 168541

De Felice S., Romanyuk Z., Chinellato M., Zoia G., Linciano S., Kumada Y., Pardon E., Steyaert J., Angelini A. and L. Cendron (2024). “Crystal structure of human serum albumin in complex with megabody reveals unique human and murine cross-reactive binding site”. Protein Science. 33, e4887

Pavan A., Cendron L., Di Nisio A., Pedrucci F., Sabovic I., Scarso A., Ferlin A., Angelini A., Foresta C. and L. De Toni (2023). “In vitro binding analysis of legacy-linear and new generation-cyclic perfluoro-alkyl substances on sex hormone binding globulin and albumin suggests low impact on serum hormone kinetics of testosterone”. Toxicology. 500, 153664

Mazzocato Y., Perin S., Morales-Sanfrutos J., Romanyuk Z., Pluda S., Acquasaliente L., Borsato G., De Filippis V., Scarso A. and A.Angelini (2023). “A novel genetically-encoded bicyclic peptide inhibitor of human urokinase-type plasminogen activator with better cross-reactivity toward the murine orthologue”. Bioorganic & Medicinal Chemistry. 95, 117499

Zorzan M., Castellan M., Gasparetto M., Zecchin B., De Melo G.D., Zecchin B., Leopardi S., Chen A., Rosato A., Angelini A., Bourhy H., Corti D., Cendron L. and P. De Benedictis (2023). “Antiviral mechanisms of two broad-spectrum monoclonal antibodies for rabies prophylaxis and therapy”. Frontiers in Immunology. 14, 1186063

Moro G., Ferrari L., Angelini A. and F. Polo (2023). "An impedimetric biosensing strategy based on bicyclic peptides bioreceptor for monitoring huPA cancer biomarker". Chemosensors. 11, 234

Kumada Y., Tanibata R., Yamamoto K., Noguchi H., Angelini A. and J. Horiuchi (2023). “Development and characterization of a latex turbidimetric immunoassay using rabbit anti-CRP single-chain Fv antibodies”. Journal of Immunological Methods. 50, 113522

Maso L., Vascon F., Chinellato M., Goormaghtigh F., Bellio P., Van Melderen L., Ruzzene M., Pardon E., Angelini A., Celenza G., Steyaert J., Tondi D. and L. Cendron (2022). “Nanobodies targeting LexA autocleavage disclose a novel suppression strategy of SOS-response pathway”. Structure. 30, 1479-93

Moro G., Liberi S., Vascon F., Linciano S., De Felice S., Foresta C., De Toni L., Di Nisio A., Cendron L. and A. Angelini (2022). “Investigation of the interaction between human serum albumin and branched short-chain perfluoroalkyl compounds”. Chemical Research in Toxicology. 35, 2049-58

Habeshian S., Merz M., Sangouard G., Mothukuri G., Schüttel M., Vesin J., Bortoli Chapalay J., Turcatti G., Cendron L., Angelini A. and C. Heinis (2022). “Accessing large macrocycle diversities by combinatorial late-stage modification at picomole scale”. Nature Communications. 13, 3823

Moro G., Sfragano P.S., Ghirardo J., Mazzocato Y., Angelini A., Palchetti I. and F. Polo (2022). “Bicyclic peptide-based assay for uPA cancer biomarker”. Biosensors and Bioelectronics. 213, 114477

Linciano S., Moro G., Zorzi A. and A. Angelini (2022). “Molecular analysis and applications of human serum albumin-fatty acid interactions”. Journal of Controlled Release. 348, 115-26

Minisini M., Di Giorgio E., Kerschbamer E., Dalla E., Faggiani M., Franforte E., Meyer-Almes F, Ragno R., Antonini L., Mai A., Fiorentino F., Rotili D., Chinellato M., Perin S., Cendron L., Weichenberger C., Angelini A. and C. Brancolini (2022). “Transcriptomic and genomic studies classify NKL54 as a histone deacetylase inhibitor with indirect influences on MEF2-dependent transcription”. Nucleic Acid Research. 50, 2566-86

Liberi S., Linciano S., Moro G., De Toni L., Cendron L. and A. Angelini (2022). “Structural analysis of human serum albumin in complex with the fibrate drug gemfibrozil”. International Journal of Molecular Sciences. 23, 1769

Linciano S., Wong E.L., Mazzocato Y., Chinellato M., Scaravetti T., Caregnato A., Cacco V., Romanyuk Z. and A. Angelini (2022). “Guidelines, strategies and principles for the directed evolution of crossreactive antibodies using yeast surface display technology”. Methods in Molecular Biology. 2491, 251-62

Pluda S., Mazzocato Y. and A. Angelini (2021). “Peptide-based inhibitors of ADAM and ADAMTS metalloproteinases”. Frontiers in Molecular Biosciences. 8, 703715

Maso L., Trande M., Liberi S., Moro G., Daems E., Linciano S., Sobott F., Covaceuszach S., Cassetta A., Fasolato S., Moretto L.M., De Wael K., Cendron L. and A. Angelini (2021). “Unveiling the binding mode of perfluorooctanoic acid to human serum albumin”. Protein Science. 30, 830-41

Daems E., Moro G., Berghmans H., Moretto L.M., Dewilde S., Angelini A., Sobott F. and K. De Wael (2021). “Native mass spectrometry for the design and selection of protein bioreceptors for perfluorinated compounds”. Analyst. 146, 2065-73

Mothukuri G.K., Kale S.S., Stenbratt C.L., Zorzi A., Vesin J., Bortoli Chapalay J., Deyle K., Turcatti G., Cendron L., Angelini A. and C. Heinis (2020). “Macrocycle synthesis strategy based on step-wise "adding and reacting" three components enables screening of large combinatorial libraries”. Chemical Science. 11, 7858-63

Moro G., Bottari F., Liberi S., Covaceuszach S., Cassetta A., Angelini A., De Wael K. and L.M. Moretto (2020). “Covalent immobilization of delipidated human serum albumin on poly(pyrrole-2-carboxylic) acid film for the impedimetric detection of perfluorooctanoic acid”. Bioelectrochemistry. 134, 107540

Mesin L., Schiepers A., Ersching J., Barbulescu A., Battaglioni Cavazzoni C., Angelini A., Okada T., Kurosaki T. and G. D. Victora (2020). “Restricted clonality and limited germinal center reentry characterize memory B cell reactivation by boosting”. Cell. 180, 92-106

Linciano S., Pluda S., Bacchin A. and A. Angelini (2019). “Molecular evolution of peptides by yeast surface display technology”. Medicinal Chemistry Communications. 10, 1569-80

Kale S.S., Bergeron-Brlek M., Wu Y., Kumar M.G., Pham M.V., Chapalay J.B., Vesin J., Kong X., Machado J.F., Deyle K., Gonschorek P., Turcatti G., Cendron L., Angelini A. and C. Heinis (2019). “Thiol-to-amine cyclization reaction enables screening of large libraries of macrocyclic compounds and the generation of sub-kilodalton ligands”. Science Advances. 5, eaaw2851

Zorzi A., Linciano S. and A. Angelini (2019). “Non-covalent albumin-binding ligands to extend the circulating half-life of small biotherapeutics”. Medicinal Chemistry Communications. 10, 1068-81

Angelini A., Miyabe Y., Newsted D., Kwan B.H., Kelly R.L., Miyabe C., Jamy M.N., Luster A.D. and K.D. Wittrup (2018). “Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in a murine model of arthritis”. Nature Communications. 9, 1461

Kwan B.H., Zhu E.F., Tzeng A., Sugito H.R., Eltahir, A.A., Ma B., Delaney M.K., Murphy P.A., Kauke M.J., Angelini A., Maragh A.M., Hynes R.O., Dranoff G., Cochran, J.R. and K.D. Wittrup (2017). “Integrin-targeted cancer immunotherapy elicits protective adaptive immune responses”. Journal of Experimental Medicine. 214, 1679-90

Pasqual G., Angelini A. and G.D. Victora (2015). “Triggering positive selection of germinal centre B cells by antigen targeting to DEC-205”. Methods in Molecular Biology. 1291, 125-34

Angelini A., Chen T.F., De Picciotto S., Yang N.J., Tzeng A., Santos M.S., Van Deventer J.A., Traxlmayr M.W. and K.D. Wittrup (2015). “Protein engineering and selection using yeast surface display”. Methods in Molecular Biology. 1319, 3-36

Zhu E.F., Gai S.A., Opel C.F., Kwan B.H., Surana R., Mihm M.C., Kauke M.J., Moynihan K.D., Angelini A., Williams R.T., Stephan M.T., Kim J.S., Yaffe M.B., Irvine D.J., Weiner L.M., Dranoff G., K.D. Wittrup (2015). “Synergistic innate and adaptive immune response to combination immunotherapy with anti-tumor antigen antibodies and extended serum half-life IL-2”. Cancer Cell. 27, 489-501

Burg J.S., Ingram J.R., Venkatakrishnan A.J., Jude K.M., Dukkipati A., Feinberg E.N., Angelini A., Waghray D., Dror R.O., Ploegh H.L. and K.C. Garcia (2015). “Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor”. Science. 347, 1113-7

Pollaro L., Raghunathan S., Morales-Sanfrutos J., Angelini A., Kontos S. and C. Heinis (2015). ”Bicyclic peptides conjugated to an albumin-binding tag diffuse efficiently into solid tumors”. Molecular Cancer Therapeutics. 14, 151-61

Chen S., Bertoldo D., Angelini A., Pojer F. and C. Heinis (2014). “Peptide ligands stabilized by small molecules". Angewandte Chemie International Edition. 53, 1602-6

Adalsteinsson V., Tahirova N., Tallapragada N., Yao X., Campiom L., Angelini A., Douce T., Huang C., Kwon D., Wittrup K.D. and J.C. Love (2013). “Single cells from human primary colorectal tumors exhibit polyfunctional heterogeneity in secretions of ELR+ CXC chemokines”. Integrative Biology. 5, 1272-81

Rentero Rebollo I., Angelini A. and C. Heinis (2013). “Phage display libraries of differently sized bicyclic peptides”. Medicinal Chemistry Communications. 4, 145-50

Angelini A., Morales-Sanfrutos J., Diderich P., Chen S. and C. Heinis (2012). “Bicyclization and tethering to albumin yields long-acting peptide antagonists”. Journal of Medicinal Chemistry. 55, 10187-97

Angelini A., Diderich P., Morales-Sanfrutos J., Thurnheer S., Hacker D., Menin L. and C. Heinis (2012). “Chemical macrocyclization of peptides fused to antibody Fc fragments”. Bioconjugate Chemistry. 23, 1856-63

Salvi N., Buratto A., Bornet A., Ulzega S., Rentero Rebollo I., Angelini A., Heinis C. and G. Bodenhausen (2012). “Boosting the sensitivity of ligand-protein screening by NMR of long-lived states”. Journal of the American Chemical Society. 134, 11076–9

Pollaro L., Diderich P., Angelini A., Bellotto S., Wegner H. and C. Heinis (2012). “Measuring net protease activities in biological samples using selective peptidic inhibitors”. Analytical Biochemistry. 427, 18-20

Chen S., Morales-Sanfrutos J., Angelini A., Cutting B. and C. Heinis (2012). “Structurally diverse cyclisation linkers impose different backbone conformations in bicyclic peptides”. ChemBioChem. 13, 1032-8

Angelini A., Cendron L., Chen S., Touati J., Winter G., Zanotti G. and C. Heinis (2012). “Bicyclic peptide inhibitor reveals large contact interface with a protease target”. ACS Chemical Biology. 7, 817–21

Angelini A. and C. Heinis (2011). “Post-translational modification of genetically encoded polypeptide libraries”. Current Opinion in Chemical Biology. 15, 355-61

Touati J., Angelini A., Hinner M.J. and C. Heinis (2011). “Enzymatic cyclisation of peptides with a transglutaminase”. ChemBioChem. 12, 38-42

Angelini A., Cendron L., Seydel A., Barison N., Battistutta R. and G. Zanotti (2009). “Towards the understanding of molecular aspects of Helicobacter pylori cag-PAI”. Book Title: Synchrotron Radiation and Structural Proteomics, Pan Stanford Series on Nanobiotechnology. Edited by C. Riekel and E. Pechkova

Barison N., Cendron L., Trento A., Angelini A. and G. Zanotti (2009). “The structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway-evidence of a different substrate specificity”. FEBS Journal. 276, 6227-35

Angelini A., Tosi T., Mas P., Acajjaoui S., Zanotti G., Terradot L. and D.J. Hart (2009). “Expression of Helicobacter pylori CagA domains by library-based construct screening”. FEBS Journal. 276, 816-24

Cendron L., Couturier M., Angelini A., Barison N., Stein M. and G. Zanotti (2009). “The Helicobacter pylori CagD (HP0525, Cag24) protein is essential for CagA translocation and maximal induction of interleukin-8 secretion”. Journal of Molecular Biology. 386, 204-17

Angelini A., Cendron L., Goncalves S., Zanotti G. and L. Terradot (2008). “Structural and enzymatic characterisation of HP0496, a YbgC thioesterase from Helicobacter pylori”. Proteins. 72, 1212-21

Cendron L., Tasca E., Seraglio T., Seydel A., Angelini A., Battistutta R., Montecucco C. and G. Zanotti (2007). “The crystal structure of CagS from the Helicobacter pylori pathogenicity island”. Proteins. 69, 440-3

Cendron L., Seydel A., Angelini A., Battistutta R. and G. Zanotti (2004). “Crystal structure of CagZ, a protein from the Helicobacter pylori pathogenicity island that encodes for a type IV secretion system”. Journal of Molecular Biology. 340, 881-9